nbp2 52874 show strong fus nuclear staining  (Novus Biologicals)

Journal: F1000Research

Article Title: The identification of high-performing antibodies for RNA-binding protein FUS for use in Western Blot, immunoprecipitation, and immunofluorescence

doi: 10.12688/f1000research.133220.2

Figure Lengend Snippet: Lysates of HeLa (WT and FUS KO) were prepared and 30 μg of protein were processed for Western Blot with the indicated FUS antibodies. The Ponceau stained transfers of each blot are presented to show equal loading of WT and KO lysates and protein transfer efficiency from the acrylamide gels to the nitrocellulose membrane. Antibody dilutions were chosen according to the recommendations of the antibody supplier. An exception was given for antibody GTX101810, which was titrated to 1/3000, as the signal was too weak when following the supplier’s recommendation. Antibody dilution used: NBP2-52874* at 1/1000; GTX101810 at 1/3000; GTX01039* at 1/1000; 60160-1-Ig* at 1/10000; 11570-1-AP at 1/4000; MA3-089* at 1/2000; MA5-32483** at 1/1000, ab124923** at 1/5000; ab154141* at 1/1000; ab243880** at 1/1000. Predicted band size: 53 kDa. Observed specific band size: ~70 kDa. *Monoclonal antibody; **Recombinant antibody.

Article Snippet: Bio Techne , NBP2-52874 , MAB-03520 , AB_2885157 , monoclonal , CL0190 , mouse , 1.00 , Wb, IF.

Techniques: Western Blot, Staining, Recombinant

Journal: F1000Research

Article Title: The identification of high-performing antibodies for RNA-binding protein FUS for use in Western Blot, immunoprecipitation, and immunofluorescence

doi: 10.12688/f1000research.133220.2

Figure Lengend Snippet: HeLa lysates were prepared, and IP was performed using 1.0 μg of the indicated FUS antibodies pre-coupled to protein G or protein A Sepharose beads. Samples were washed and processed for Western Blot with the indicated FUS antibody. For Western Blot, NBP2-52874* and ab243880** were used at a dilution of 1/2000. The Ponceau stained transfers of each blot are shown for similar reasons as in . SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitated. *Monoclonal antibody; **Recombinant antibody.

Article Snippet: Bio Techne , NBP2-52874 , MAB-03520 , AB_2885157 , monoclonal , CL0190 , mouse , 1.00 , Wb, IF.

Techniques: Western Blot, Staining, Immunoprecipitation, Recombinant

Journal: F1000Research

Article Title: The identification of high-performing antibodies for RNA-binding protein FUS for use in Western Blot, immunoprecipitation, and immunofluorescence

doi: 10.12688/f1000research.133220.2

Figure Lengend Snippet: HeLa WT and FUS KO cells were labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with the indicated FUS antibodies and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the red (grayscale) channels are shown. WT and KO cells are outlined with yellow and magenta dashed line, respectively. Antibody dilutions were chosen according to the recommendations of the antibody supplier. Exceptions were given for antibodies GTX101810, GTX01039*, 60160-1-Ig*, 11570-1-AP, MA5-32483** and ab124923**, which were titrated as the signals were too weak when following the supplier’s recommendations. Antibody dilution used: NBP2-52874* at 1/1000; GTX101810 at 1/700; GTX01039* at 1/1000; 60160-1-Ig* at 1/2000; 11570-1-AP at 1/1000; MA3-089* at 1/1000; MA5-32483** at 1/1000; ab124923** at 1/1000; ab154141* at 1/1000; ab243880** at 1/500. Bars=10 μm. *Monoclonal antibody; **Recombinant antibody.

Article Snippet: Bio Techne , NBP2-52874 , MAB-03520 , AB_2885157 , monoclonal , CL0190 , mouse , 1.00 , Wb, IF.

Techniques: Staining, Recombinant

Journal: F1000Research

Article Title: The identification of high-performing antibodies for RNA-binding protein FUS for use in Western Blot, immunoprecipitation, and immunofluorescence

doi: 10.12688/f1000research.133220.2

Figure Lengend Snippet: Summary of the FUS antibodies tested.

Article Snippet: Bio Techne , NBP2-52874 , MAB-03520 , AB_2885157 , monoclonal , CL0190 , mouse , 1.00 , Wb, IF.

Techniques: Concentration Assay, Recombinant